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Journal: Journal of Traditional Chinese Medicine
Article Title: Astragaloside IV plays a role in reducing radiation-induced liver inflammation in mice by inhibiting thioredoxin-interacting protein/nod-like receptor protein 3 signaling pathway
doi: 10.19852/j.cnki.jtcm.2023.01.008
Figure Lengend Snippet: A: immunofluorescence expression of NLRP3 inflammasome in each group. A1: Control group; A2: DMSO group; A3: IR group; A4: IR+AS-IV-20 group; A5: IR+AS-IV-40 group; B1: the expressions of TXNIP, NLRP3, ASC, Caspase-1 and IL-1β in liver were measured by Western blot; B2: relative density of TXNIP; B3: relative density of NLRP3; B4: relative density of ASC. B5: relative density of Caspase-1; B6: relative density of IL-1β. DMSO: dimethyl sulfoxide; IR: irradiation; IR+AS-20: irradiation+AS-IV (20 mg/kg); IR + AS-40: irradiation +AS-IV (40 mg/kg); AS-IV: astragaloside IV; TXNIP: Thioredoxin-interacting protein; NLRP3: nod-like receptor protein 3; ASC: apoptosis-associated speck-like protein containing a CARD; Caspase-1: cysteinyl aspartate-specific proteinase 1; IL-1β: interleukin 1beta. aP < 0.001 vs Control group; bP < 0.001 vs IR group; cP < 0.05 vs IR + AS-20 group; dP < 0.01 vs IR group; eP < 0.01 vs IR + AS-20 group.
Article Snippet: After blocked in 5% nonfat dry milk for 1 h, membranes were washed with Tris-buffered saline with tween 20 (TBST) for 3 × 10 min. Then, membranes were incubated with the primary
Techniques: Immunofluorescence, Expressing, Western Blot, Irradiation